Aquetong Creek supports native brown trout and Ingham Spring was used as a trout hatchery in the past. It is probable that trout reproduction occurs within Aquetong Creek because small/young trout have been observed in the creek and the dam at the Bucks County Playhouse prohibits trout from swimming upstream from the Delaware River. Other species that occur in the creek include American eel, white suckers and bass as well as northern two-lined salamanders and green frogs. A variety of turtles, including snapping and painted turtles, occur in the watershed. Eastern spiny softshell turtles are native to western Pennsylvania, but have been introduced into eastern Pennsylvania, and may occur in Ingham Lake. Macroinvertebrates include a variety of mayflies, stoneflies, caddisflies, as well as other genera.